110469     Regression Analysis for Michaelis-Menten Kinetic Data
Version: January 1981
 
Submitted by: G. C. Scott
 
Operating System: RT-11  Source Language: FORTRAN IV  Memory Required:
30K Vir.Mem.,2K Dir.Address.Mem. for array storage  Keywords:
Scientific Applications, Statistics
 
Abstract: This FORTRAN program calculates the Michaelis-Menton
parameters Vm, the maximum velocity, and Km, the
substrate concentration at half-maximal velocity, and
their 95% confidence ranges for the dependence of enzyme
activity of substrate concentration, in the absence of
product. The program uses simple non-linear regression,
non-parametric statistics, unweighted linear regression,
and iterative weighted linear regression. The program
requests the following inputs from the user: a selection
of the type(s) of regression(s) to be performed, a file
of substrate and rate data containing up to 50 data
points, and an appropriate T-statistic.
 
The initial Km bracket values for the non-linear
calculations are assumed to be BRKM(1) = 0.0 and BRKM(2)
= 10.0. If this interval does not bracket the true Km,
BRKM(2) is doubled until the appropriate bracket
interval is obtained. Any negative Vm or Km values
generated by the non-parametric calculations are
replaced by an arbitrarily large number (1.0E+6) and
entered into the sorting routine with the other smaller
and non-negative Vm and Km values. The final two values
of the iterative weighted linear sum of least squares
calculations are tested for a convergence tolerance of
5.0%.
 
Restrictions: Regression cannot be performed for more than 50 data
pairs.
 
Media (Service Charge Code): Source Listing (BA), One RX01 Diskette
(KA)  Format: RT-11, 600' Magnetic Tape (MA)  Format: RT-11